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New member of IQ2000^TM family: YHV/GAV Detection and Typing System

In 1992, yellow-head virus (YHV) was first discovered in Thailand on black tiger prawn (Penaeus monodon), although it is now known to have caused extensive losses on the eastern coast of the Gulf of Thailand as early as 1991 (Flegel et al. 1995).  This disease affects juvenile to sub-adult prawn and is usually characterized by a pale to yellowish coloration of the cephalothorax and gills, and erratic swimming of infected animal near the surface at the pond edge.  This virus caused $40 millions estimative loss in Thailand every year from 1995 till now.  Two viruses, which morphologically resemble YHV, have also been reported in lymphoid organs (LOV) (Spann et al. 1995) and gills (GAV) (Spann et al. 1998) of Penaeus monodon from Australia.  These viruses are also known to infect other species of penaeid shrimp in laboratory tests, so they are not potential pathogens solely to P. monodon.  Since GAV and YHV are RNA viruses, cDNA preparation is necessary in order to characterize their nucleic acid and to prepare the diagnostic probes.  The final aim is to develop a rapid, simple and sensitive reverse transcription polymerase chain reaction (RT-PCR) based system which would allow early detection of the virus. 

The IQ2000^TM YHV/GAV Detection and Typing System for penaeid shrimps is the first commercial molecular biology system for direct detection of these two similar viruses.  IQ2000^TM YHV/GAV Detection and Prevention System is designed by transferring the research results from both the CSIRO, Australia, and BIOTEC, Thailand.  It can differentiate these two viruses and also provide the severity information.

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