Farming IntelliGene Technology Corporation
Your Partner for Viral Detection and Prevention
|
IQ2000TMKit
|
||||||||||||||||||||||||||
|
IQ REAL System
| ||||||||||||||||||||||||||
|
P.H. Teng, C. Su, C.L. Chen, P. Y. Lee Nucleic acid-based amplification (NASBA) technology, an isothermal reaction, offers speed, sensitivity and specificity comparable to PCR for pathogen detection. Requiring only simple heating devices, it is suitable for the development of an on-site diagnosis system for aquaculture pathogens. In this report, a NASBA assay has been developed for Taura syndrome virus (TSV), one of the most devastation virus pathogens for several Penaeid shrimp species. The NASBA products were confirmed by Northern blotting and dot-blot blotting analysis. Agarose gel electrophoresis of the reaction end products indicated that NASBA and the nested PCR-based IQ2000TM TSV Detection and Prevention System reach similar degrees of sensitivity. The specificity of the TSV NASBA reaction was substantiated by the results that non-target virus samples do not generate any signals. A solid phase-based colorimetric assay employing TSV-specific capture and detection primers was developed to detect the NASBA amplicons. Taken together, the combination of NASBA and a solid phase-based colorimetric assay to identify the amplicons should allow easy and rapid TSV detection. |
|
To prevent the infection by the viruses from last season, some shrimp farmers collect the pond dirt for the virus detection. However, is it a useful strategy for the TSV prevention? There is an example. A Taiwanese shrimp farm was struck by the TSV in October 2002. After a month of pond drying, there were 5 dirt samples collected from four corners and the center of the pond for the TSV test by IQ2000TM TSV Detection and Prevention System. All of five samples had shown the negative results. At the same time, another experiment was still under progressing. This experiment was taken 2 kg of the pond dirt into an aquarium. After sedimentation, 10 pieces of the SPF L. vannamei (10 to 12 gm) were put into that aquarium. The reference group used the ordinary soils instead of the pond dirt, and also put 10 SPF animals. After 10 days, the gills were sampled and the results were TSV positive for the experimental group. This experiment has been repeated once and the same result was shown. Therefore, we could say that the pond dirt screening of TSV cannot reflect the actual infectivity. In the other hand, pure pond drying is not very useful to destroy the remained TSV. |
¡mTSV01¡n ¡mTSV02¡n
¡iProduct¡j¡iVirus Info¡j¡iNews¡j¡iService¡j¡iQ&A¡j¡iAbout us¡j¡iDistributors¡j