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Introduction
PCR technique has been commonly employed in the aquaculture industry for disease diagnosis and prevention. With the growing demand for a quantitative research tool and requirement for a high throughput screening system, real-time PCR is receiving more attention and becoming more important in the industry.
GeneReach has developed IQ REAL™ IHHNV Quantitative System, a real-time based diagnostic system. Its advanced designs, listed below, provides an accurate, sensitive, and flexible real-time system to the shrimp farming industry.
Advantages of IQ REAL™ IHHNV Quantitative System:
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TaqMan® assay strategy: This system can tolerate higher concentration of sample load than SYBR Green. Consequently, it is able to detect the samples with very low viral/shrimp cell ratio.
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Dual color design: This system can quantify both viral (FAM) and shrimp genomic nucleic acid (VIC) by a single reaction. This built-in internal control avoids the false negative resμlts from failed extraction or inhibition.
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Absolute and comparative quantitation: This system can quantify the viral amount by both absolute quantitative method (Qab, viral amount/μl) and comparative quantitative method (Qcp, viral amount/ 10,000 shrimp cells). The Qcp method can reflect the real infectious level and provide constant result for a sample within wide range of concentration.
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No standard required: There is no need to make a calibration curve for Qcp method. Since a unique ΔΔCt strategy is introduced to interpret the final result , there will be no waste for standards, as the reagents are only for the use of samples.
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