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Real-time PCR system for detection of Marine Fish Iridovirus

 

Introduction

PCR technology has been commonly employed in the aquaculture industry for disease diagnosis and prevention. But with the growing demands for a quantitative research tool and requirement for a high throughput screening system, real-time PCR is receiving more attention and becoming more important in the industry than before. GeneReach has developed IQ REAL™ Irido Quantitative System a real-time PCR-based diagnostic system. Its advanced designs, listed below, provide an accurate, sensitive, and flexible real-time PCR system for iridovirus detection in the fish culture industry.

Advantages of IQ REAL™ Irido Quantitative System:

  1. TaqMan® assay strategy: This system can tolerate higher sample loads than SYBR Green based reaction. Consequently, it is able to detect samples of very low target/fish cell ratio.
  2. Dual color design: This system can quantify both target pathogen (FAM) and fish (VIC) genomic nucleic acid in a single reaction. The built-in internal control (VIC) prevents any false negative interpretation of results from failed extraction or inhibition.
 
 
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