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Real-time PCR system for detection of Nervous Necrosis Virus (VNN)



PCR technology has been commonly employed in the aquaculture industry for disease diagnosis and prevention. With the growing demands for a quantitative research tool and requirement for a high throughput screening system, real-time PCR is receiving more attention and becoming more important in the industry than before.

GeneReach has developed IQ REAL™ VNN Quantitative System, a real-time PCR-based diagnostic system. Its advanced designs, listed below, provides an accurate, sensitive, and flexible real-time PCR system for VNN detection in the fish farming industry.

Advantages of IQ REAL™ VNN Quantitative System:

  1. TaqMan® assay strategy: This system can tolerate higher sample loads than SYBR Green based reaction. Consequently, it is able to detect samples of very low target/fish cell ratio.
  2. Dual color design: This system can quantify both target pathogen (FAM) and fish (VIC) genomic nucleic acid in a single reaction. The built-in internal control (VIC) prevents any false negative interpretation of results due to failed extraction or inhibition.
  3. Absolute and comparative quantitation: This system can quantify the target pathogen by both absolute quantitation method (Qab, target quantity/μl) and comparative quantitation method (Qcp, target quantity/10,000 fish cells). The Qcp method can reflect the actual infection level and provide consistent results for a sample within a wide range of concentration.
  4. No standard required for Qcp method: There is no need to make a calibration curve for Qcp method. Since the ΔΔCt strategy is used to interpret the final results, there is no need to generate a standard curve in each run, saving reagent costs in the long run.
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