Hepatopancreatic parvovirus (HPV) has been reported in many species of penaeid shrimps worldwide and can cause poor growth and reduced yield for shrimp farmers. Infections by HPV produce an intranuclear inclusion body which is mostly distributed in the distal portion of the hepatopancreatic tubules. The diagnosis of HPV traditionally depended on histological methods and transmission electron microscopy (TEM). Both methods provide reliable results but to perform the test, it requires specialists and sophisticated equipment which makes it impractical for routine diagnosis. Furthermore, the techniques applied for both methods are invasive as they require destruction of the infected organs, which will inevitably sacrifice valuable stocks.
On the contrary, detection of HPV by polymerase chain reaction (PCR) provides a non-invasive alternative for the industry. PCR-based assay has several advantages such as high throughput, accuracy and high sensitivity. Owing to the high sensitivity of PCR assay, DNA extracted from the feces of broodstocks can serve as PCR template and no broodstocks will be sacrificed during sampling process.
The advantage of choosing IQ2000™ HPV Detection and Prevention System is its unique ability to detect different HPV strains from different areas, such as those from Thailand, Australia, Korea and Taiwan etc. The conserved segment of HPV is amplified by the exquisite designed primer set and the severity of infection can be distinguished easily from the electrophoresis pattern of PCR product same as our other IQ2000™ serial products. IQ2000™ HPV Detection and Prevention System provides you the most accurate, reliable and economical diagnosis tool.
Packing size: 200 reactions.
Detection Limit: 100 copies/reaction.
Quantitative capability: 3 different levels of infection can be differentiated.
Sample throughput: for 40 samples, from sample preparation to final results require 2.5 to 3 hours.
Built-in internal control system: eliminate false negative results from failed extraction or reaction.
Quantified positive standard: monitor the sensitivity of detection.
An example of the results is shown and explained below:
Lane 1: HPV P(+) standard, 20,000 copies/reaction
Lane 2: HPV P(+) standard, 2,000 copies/reaction
Lane 3: HPV P(+) standard, 200 copies/reaction
Lane 4: Negative control (Yeast tRNA or ddH2O)
Lane 5: Sample of moderate HPV infection
Lane 6: Sample of light HPV infection
Lane 7: HPV negative sample
Lane M: Molecular weight markers, 848 bp, 630 bp, 333 bp
This reagent is intended for testing fresh, frozen, and ethanol-preserved samples, e.g. broodstock’s feces, PL and hepatopancreas.
Ideal for the specific pathogen-free (SPF) animal selection, evaluation of culture environment, grow-out monitoring, and quarantine service.