The specificity of IQ2000 IHHNV nested Detection and Prevention System has been proved. 
1.  This kit can specifically detect the IHHNV isolated from different geographic area.
2.  This kit can specifically pick up the infectious IHHNV without interacting with the integrated IHHNV in the P. monodon.
    Dr. Kathy Tang-Nelson from the University of Arizona tested IQ2000 IHHNV nested Detection and Prevention System (a nested PCR with sensitivity of 10 copies/reaction ) on the specificity of this kit with samples of (1) IHHNV isolates, (2) P. monodon DNA containing IHHNV-related sequences, and (3) DNA extracted from SPF penaeids and aquatic organisms that were used as feed for shrimp culture.
    The result showed that there were no non-specific reactions with 13 samples of SPF penaeids, lobster, crabs, artemia, krill, polychaetes. This PCR kit did not react to the 42 samples P. monodon containing integrated IHHNV-related sequences that were collected from Tanzania, Australia, shrimp farms in Malaysia, and India. Also, this kit is specific to IHHNV and reacts to purified IHHN virions and IHHNV isolates from America (Hawaii, Brazil, Belize, Mexico), Oceania, and SE Asia. There is no cross reaction to another shrimp parvo-like virus, HPV. 
    Users who concern about integrated IHHNV and need higher analytical sensitivity can choose this kit to fit for their detection purposes. However, IQ2000 IHHNV Detection and Prevention System (one step PCR with sensitivity of 100 copies/reaction) is still available for users who only detect IHHNV on penaeid shrimp other than P. monodon.

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