IQ 2000
IQ REAL
IQ Plus
 
  鯉魚春季毒血症病毒 (SVCV)

Home  /  Products  /  IQ REAL  /  鯉魚春季毒血症病毒 (SVCV)

Real-time PCR system for detection of Spring Viremia of Carp Virus (SVCV)

 

Introduction

PCR technique has been commonly employed in the aquaculture industry for disease diagnosis and prevention. But with the growing demand for a quantitative research tool and requirement for a high throughput screening system, real-time PCR is receiving more attention and becoming important in the industry. IQ REAL SVCV Quantitative System is a real-time based diagnostic system which not only meets the above demands but its advanced designs, listed below, also provides the shrimp culture industry a more accurate, more sensitve and more flexible real-time system.

Advantages of IQ REAL SVCV Quantitative System :

  1. TaqMan® assay strategy: This system can tolerate higher concentration of sample load than SYBR Green. Consequently, it is able to detect the samples with very low viral/fish cell ratio.
  2. Dual color design: This system can quantify both viral (FAM) and shrimp genomic nucleic acid (VIC) by a single reaction. This built-in internal control avoids the false negative results from failed extraction or inhibition.
  3. Absolute and comparative quantitation: This system can quantify the viral amount by both absolute quantitative method (Qab, viral amount/µl) and comparative quantitative method (Qcp, viral amount/ 10,000 shrimp cells). The Qcp method can reflect the real infectious level and provide constant result for a smaple within wide range of concentration.
  4. No standard required: There is no need to make a calibration curve for Qcp method. A unique ΔΔCt strategy is introduced to interpret the final result. There is no waste for standards as the reagents are only for the use of samples.
  • TaqMan® is the trademark licensed under Applied Biosystems.

 

 
 

關於我們  |  最新消息  |  產品資訊  |  服務項目  |  聯絡我們  |  首頁
Copyright © 2015 GeneReach Biotechnology Corp. All rights reserved.    E-mail : sales@genereachbiotech.com
Designed by Dtell網頁設計