Spring Viremia of Carp (SVCV) Detection and Prevention for Cyprinid Fish

Introduction:

Spring Viremia of Carp (SVC) is a contagious viral disease affecting cyprinid fish, including common carp, koi carp, grass carp and other related species. The disease is caused by the spring viremia of carp virus (SVCV). It belongs to Rhabdoviridae and is a bullet-shaped RNA virus. It enters the fish most often through the gills and replicates in gill epithelium. Because of the longevity of the virus in water, mud, or following desiccation, transmission is thought to be by direct contact or through the water. SVCV isolates can be divided into four genetic groups. And the primer sets in the IQ2000^TM SVCV Prevention and Detection Kit was designed to detect the genogroup Ia which originate from Asia.

The IQ2000^TM SVCV Prevention and Detection Kit has adopted the design of Nested PCR and also inherited the reliability, sensitivity and experience from a range of IQ2000^TM virus diagnostic kits for shrimp and fish virus disease. Its unique semi-quantitative function provides information of virus infection levels which could be used for further understanding on SVCV.

Specifications:

 Packing size: 200 reactions.

 Detection Limit: 10 copies/reaction.

 Quantitative capability: 3 different levels of infection can be differentiated.

 Sample throughput: for 40 samples, from sample preparation to final results require 3.5 to 4 hours.

 Built-in internal control system: eliminate false negative results from failed extraction or reaction.

 Quantified positive standard: monitor the sensitivity of detection.

Typical diagnostic results generated by IQ2000^TMSVCV Detection and Prevention System.

Lane 1: SVCV P(+) standard, 2000 copies/reaction

Lane 2: SVCV P(+) standard, 200 copies/reaction

Lane 3: SVCV P(+) standard, 20 copies/reaction

Lane 4: Negative control (Yeast tRNA)

Lane 5: Sample of severe SVCV infection

Lane 6: Sample of moderate SVCV infection

Lane 7: Sample of light SVCV infection

Lane 8: SVCV negative sample

Lane 9: Molecular weight marker, 848 bp, 630 bp, 333 bp

Applications:

 Suitable for fresh, frozen and ethanol preserved fish tissue samples, e.g. larvae, gill, kidney and spleen.

 Ideal for SPF animal selection, grow-out monitoring, and quarantine service.

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