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candy_blue.gif Preparation

  • What's the difference between the Lysis Buffer and DTAB/CTAB solution?...(more)
  • Should I grind the shrimp tissues very hard to recover more DNA?...(more)
  • Which shrimp organ may contain PCR inhibitors?...(more)
  • Will the DNA concentration affect PCR results? What's the ideal DNA concentration for PCR?...(more)

candy_blue.gif Preservation

  • What are the better ways to preserve shrimp samples?...(more)
  • What care should be taken when we preserved samples by ethanol?...(more)
  • Can I preserve the samples in Lysis Buffer or DTAB solution instead of ethanol?...(more)
  • Can we use TE buffer instead of ddH2O to dissolve the DNA pellet?...(more)
  • Can I preserve my samples with davidson's fixative?...(more)

candy_blue.gif Sampling

  • What's the best organ of adult shrimps for WSSV diagnosis...(more)
  • What's the best period for brooder diagnosis...(more)
  • How many PLs should we sample to reach enough confidence level...(more)
  • In the grow-out pond monitoring, how often should we do the diagnosis? How many shrimps should we take while sampling?...(more)

candy_blue.gif PCR procedure

  • What dose "PCR negative results" mean...(more)
  • How do I know my PCR cycler is operating under proper condition...(more)
  • What's the advantage of the nested PCR...(more)
  • How do I avoid false positive and false negative results from PCR...(more)
  • How do I know the sensitivity is approprate? Can I adjust the sensitivity...(more)
  • Must I see the house-keeping gene's product from the negative samples..(more)
  • Sometimes I can not get the house-keeping gene from negative samples. How can I improve..(more)
  • Why did I get the smear pattern from the gel electrophoresis..(more)
  • Why did I get the non-specific bands..(more)
  • How do I avoid the PCR contamination problem..(more)
  • I have to do the WSSV and TSV detection in my daily life. Is it possible that I can make it simultaneously? I don't want to spend my time on sitting and waiting for the results....(more) words_sp_new.gif

candy_blue.gif Others

  • How to monitor the epidemic risk in the culture environment..(more)
  • What should we do if we get a positive result for virus infection in our shrimps..(more)

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