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 Preparation
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- What's the difference between the Lysis Buffer and DTAB/CTAB solution?...(more)
- Should I grind the shrimp tissues very hard to recover more DNA?...(more)
- Which shrimp organ may contain PCR inhibitors?...(more)
- Will the DNA concentration affect PCR results? What's the ideal DNA concentration for PCR?...(more)
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Preservation
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- What are the better ways to preserve shrimp samples?...(more)
- What care should be taken when we preserved samples by ethanol?...(more)
- Can I preserve the samples in Lysis Buffer or DTAB solution instead of ethanol?...(more)
- Can we use TE buffer instead of ddH2O to dissolve the DNA pellet?...(more)
- Can I preserve my samples with davidson's fixative?...(more)
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Sampling
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- What's the best organ of adult shrimps for WSSV diagnosis...(more)
- What's the best period for brooder diagnosis...(more)
- How many PLs should we sample to reach enough confidence level...(more)
- In the grow-out pond monitoring, how often should we do the diagnosis? How many shrimps should we take while sampling?...(more)
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PCR procedure
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- What dose "PCR negative results" mean...(more)
- How do I know my PCR cycler is operating under proper condition...(more)
- What's the advantage of the nested PCR...(more)
- How do I avoid false positive and false negative results from PCR...(more)
- How do I know the sensitivity is approprate? Can I adjust the sensitivity...(more)
- Must I see the house-keeping gene's product from the negative samples..(more)
- Sometimes I can not get the house-keeping gene from negative samples. How can I improve..(more)
- Why did I get the smear pattern from the gel electrophoresis..(more)
- Why did I get the non-specific bands..(more)
- How do I avoid the PCR contamination problem..(more)
- I have to do the WSSV and TSV detection in my daily life. Is it possible that I can make it simultaneously? I don't want to spend my time on sitting and waiting for the results....(more)
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Others
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- How to monitor the epidemic risk in the culture environment..(more)
- What should we do if we get a positive result for virus infection in our shrimps..(more)
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