Necrotizing hepatopancreatitis bacteria Detection and Prevention for Shrimp Farming (NHP)

Introduction:

Necrotizing hepatopancreatitis (NHP) is a bacterial disease which targets penaeid shrimp. NHP was originally identified in 1990 in cultured Litopenaeus vannamei in Texas, USA (Frelier et al. 1992). The disease has now been reported in several locations throughout the Western hemisphere. NHPB can result in significant production losses, with mortality losses ranging from 20% to 95% within 30 days of an outbreak.

NHPB is a gram-negative, pleomorphic, intracellular, rickettsia-like bacterium. It is necessary to use molecular methods for phylogenetic placement of the NHPB due to the inability of culturing it. The research for NHPB is continuously on going. It is recognized that the disease is not transmitted vertically.

In cooperation with the research team led by Dr. Donald V. Lightner, Farming IntelliGene Tech Corp. has developed a highly sensitive NHP diagnosis system - IQ2000^TM NHPB Detection and Prevention System. This system has adopted the design of Nested PCR and can differentiate infected shrimps into four levels: severe, moderate, light and very light.

Specifications:

 Packing size: 200 reactions.

 Detection Limit: 10 copies/reaction.

 Quantitative capability: 4 different levels of infection can be differentiated.

 Sample throughput: for 40 samples, from sample preparation to final results require 4.5 to 5 hours.

 Built-in internal control system: eliminate false negative results from failed extraction or reaction.

 Quantified positive standard: monitor the sensitivity of detection.

 Adaptable to the Uni-IQ RT-PCR profile, suitable for multi-viral diagnosis.

Typical diagnostic results generated by IQ2000^TM NHPB Detection and Prevention System.

Lane 1: NHPB P(+) standard, 2000 copies/reaction

Lane 2: NHPB P(+) standard, 200 copies/reaction

Lane 3: NHPB P(+) standard, 20 copies/reaction

Lane 4: Negative control (yeast tRNA or ddH₂O)

Lane 5: Sample of severe NHPB infection

Lane 6: Sample of moderate NHPB infection

Lane 7: Sample of light NHPB infection

Lane 8: Sample of very light NHPB infection

Lane 9: NHPB negative sample

Lane M: Molecular weight marker, 848 bp, 630 bp, 333 bp

Applications:

 Suitable for fresh, frozen and ethanol preserved shrimp tissue samples, e.g. broodstock's feces, hepatopancreas and PL.

 Ideal for SPF animal selection, evaluation of culture environment, grow-out monitoring, and quarantine service.

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