Penaeus monodon-type Baculovirus Detection and Prevention For Shrimp Farming (MBV)

Introduction:

Penaeus monodon-type baculovirus (MBV) is one of the earliest found shrimp viruses, but its impact on shrimp farming has yet to be further studied.  MBV was considered to be one of the most critical fatal shrimp diseases for P. monodon in the past, but recent researches have shown no direct association of such disease to the cause of larger failure of shrimp culture industry in respect of growth retardation, deformation, and mortality rate.

Nevertheless, MBV is still a considerable issue for hatchery management as it may affect the survival rate of PLs.  The consequences of severe MBV infection not simply affect the PLs survival rate, but the performance of PLs also turns out to be worse than those lightly infected or negative ones.  As the major transmission path of MBV is horizontal, the status of PLs MBV infection has been taken as a major indicator of hatchery hygiene in some areas.

The comparison of MBV infected samples from several Asian countries has indicated that the variation of nucleic acid sequence is higher than expected.  For example, within the published sequence by Craig R. Belcher (J. Virol. Method 74, 1998, p21-29), the homology of the sample from Taiwan is only 91.8%; 92.3% for sample from Thailand, 92.1% for sample from Indonesia, and 94.1% for sample from India.  These sequence variations may cause false negative results by following Craig's protocol.

Farming IntelliGene has been researching on MBV continuously.  This newly released version of IQ2000^TM MBV Detection and Prevention System has the broadest coverage of strains in comparison with other known products in the market and has also inherited the built-in internal control design and semi-quantitative data format from a chain of our IQ2000^TM series products.  Moreover, we always ensure that our detection system is updated as soon as we have any advanced research results in order to provide the best detection tool for the users of IQ2000^TM series products.

Specifications:

1. Packing size: 200 reactions.

2. Detection Limit: 100 copies/reaction.

3. Quantitative capability: 3 different levels of infection can be differentiated.

4. Sample throughput: for 40 samples, from sample preparation to final results require 2.5 to 3 hours.

5. Built-in internal control system: eliminate false negative results from failed extraction or reaction.

6. Quantified positive standard: monitor the sensitivity of detection.

Typical diagnostic results generated by IQ2000^TM MBV Detection and Prevention System.

Lane 1: MBV P(+) standard, 20000 copies/reaction

Lane 2: MBV P(+) standard, 2000 copies/reaction

Lane 3: MBV P(+) standard, 200 copies/reaction

Lane 4: Negative control (yeast tRNA or ddH2O)

Lane 5: Sample with severe MBV infection

Lane 6: Sample with light MBV infection

Lane 7: Fecal sample with MBV infection

Lane 8: MBV negative sample

Lane M: Molecular weight marker, 848 bp, 630 bp, 333 bp

Applications:

• Suitable for fresh, frozen, and ethanol preserved shrimp tissue samples, e.g. broodstock's feces, PL and hepatopancreas.

• Ideal for SPF animal selection, evaluation of culture environment, grow-out monitoring, and quarantine service.

go to top