Koi Herpesvirus Detection and Prevention for Koi carp Farming (KHV)

Introduction:

Koi carp has long been regarded as an important tropical ornamental fish of very high added value in the fish farming industry in Asia.  In recent years, the industry has been suffering from a severe blow through the infection of Koi Herpesvirus—KHV.  The outbreak of KHV is found in some Asia countries, such as Taiwan, Hong Kong and Indonesia, as well as in Israel and Europe.   KHV disease process is highly contagious and is most characteristic by causing massive morbidity and mortality rate ranging between 80%-90%.  According to an Indonesianresearch analysis (Agus Sunarto et. al, paper presented in 5^th Symposium on Disease in Asian Aquaculture, 14-28 Nov. 2002, Gold Coast, Australia), the process of KHV infection in East Java has affected over 5000 fish farmers and caused a loss of US$0.5 million from March to October in 2002.   As the trading business of ornamental fish among Asian countries has been very popular, the prevention of KHV exposure and quarantine is no doubt a key issue in the protection of local koi farming industry.

IQ2000^TM KHV Detection and Prevention System is the first viral diagnostic system specially designed for ornamental fish by Farming IntelliGene.  This system has adopted the design of Nested PCR and also inherited the reliability, sensitivity and experience from a range of IQ2000^TM viral diagnostic kits for shrimp and fish viral infections. IQ2000^TM KHV has effective and reproducible detection limit of 10 viral copies while in most cases digit copy of virus can also be detected. IQ2000^TM KHV offers you the best tool for the prevention of KHV infection.

Specifications:

1. Packing size: 200 reactions.

2. Detection Limit: 10 copies/reaction.

3. Quantitative capability: 3 different levels of infection can be differentiated.

4. Sample throughput: for 40 samples, from sample preparation to final results require 3.5 to 4 hours.

5. Built-in internal control system: eliminate false negative results from failed extraction or reaction.

6. Quantified positive standard: monitor the sensitivity of detection.

7. Adaptable to the Uni-IQ RT-PCR profile, suitable for multi-viral diagnosis.

Typical diagnostic results generated by IQ2000^TM KHV Detection and Prevention System.

Lane 1: KHV P(+) standard, 2000 copies/reaction

Lane 2: KHV P(+) standard, 200 copies/reaction

Lane 3: KHV P(+) standard, 20 copies/reaction

Lane 4: Negative control (yeast tRNA or ddH₂O)

Lane 5: Sample with severe KHV infection

Lane 6: Sample with light KHV infection

Lane 7: KHV negative sample

Lane M: Molecular weight marker, 848 bp, 630 bp, 333 bp

Applications:

• Suitable for fresh, frozen and ethanol preserved fish tissue samples, e.g. larvae, gill and swab.

• Ideal for SPF animal selection, evaluation of culture environment, grow-out monitoring, and quarantine service.

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