Iridovirus Detection and Prevention for Marine Fish Farming (Iridovirus)

Introduction:

Iridoviruses have caused ecological and economic impacts on marine fish farming in recent years. Pathogenic Iridovirus was isolated from diseased grouper (Epinephelus hybrid), giant seaperch (Lates calcarifer), largemouth bass (Micropterum salmoides), king grouper (Epinephelus lanceolatus), spotted butter fish (Scatophagus argus), yellow-wax pomfrat (Trachinotus blochii), goldlined seabream (Sparus sarba), humpback grouper (Chromileptes altivelis) and Mangrove red snapper (Lutjanus argentimaculatus). The virus causes severe mortality in cultured grouper fry.

IQ2000^TM Irido Detection and Prevention System is the first viral diagnostic system specially designed for marine fish by Farming IntelliGene.  This system has adopted the design of Nested PCR and also inherited the reliability, sensitivity and experience from a range of IQ2000^TM viral diagnostic kits for shrimp and fish viral infections.  IQ2000^TM Irido has an effective and reproducible detection limit of 10 viral copies while in most cases digit copy of virus can also be detected.  IQ2000^TM irido offers you the best tool for the prevention of Iridovirus infection.

Specifications:

1. Packing size: 200 reactions.

2. Detection Limit: 10 copies/reaction.

3. Quantitative capability: 3 different levels of infection can be differentiated.

4. Sample throughput: for 40 samples, from sample preparation to final results require 3.5 to 4 hours.

5. Built-in internal control system: eliminate false negative results from failed extraction or reaction.

6. Quantified positive standard: monitor the sensitivity of detection.

7. Adaptable to the Uni-IQ RT-PCR profile, suitable for multi-viral diagnosis.

Typical diagnostic results generated by IQ2000^TM Irido Detection and Prevention System.

Lane 1: Iridovirus P(+) standard, 2000 copies/reaction

Lane 2: Iridovirus P(+) standard, 200 copies/reaction

Lane 3: Iridovirus P(+) standard, 20 copies/reaction

Lane 4: Sample of severe Iridovirus infection

Lane 5: Sample of light Iridovirus infection

Lane 6: Iridovirus negative sample

Lane 7: Negative control (yeast tRNA or ddH₂O)

Lane M: Molecular weight marker, 848 bp, 630 bp, 333 bp

Applications:

• Suitable for fresh, frozen and ethanol preserved fish tissue samples, e.g. larvae, gill and swab.

• Ideal for SPF animal selection, evaluation of culture environment, grow-out monitoring, and quarantine service.

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