Real-Time PCR System for Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV)

Introduction:

PCR technique has been commonly employed in the aquaculture industry for disease diagnosis and prevention. With the growing demand for a quantitative research tool and requirement for a high throughput screening system, real-time PCR is receiving more attention and becoming more important in the industry.

Farming IntelliGene has developed IQ REAL™ IHHNV Quantitative System, a real-time based diagnostic system. It not only meets the demands, but its advanced designs, listed below, also provides a more accurate, more sensitive, and more flexible real-time system to the shrimp farming industry.

Advantages of IQ REAL^TM IHHNV Quantitative System

1. TaqMan^® assay strategy: This system can tolerate higher concentration of sample load than SYBR Green. Consequently, it is able to detect the samples with very low viral/shrimp cell ratio.

2. Dual color design: This system can quantify both viral (FAM) and shrimp genomic nucleic acid (VIC) by a single reaction. This built-in internal control avoids the false negative resμlts from failed extraction or inhibition.

3. Absolute and comparative quantitation: This system can quantify the viral amount by both absolute quantitative method (Q_ab, viral amount/μl) and comparative quantitative method (Q_cp, viral amount/10,000 shrimp cells). The Q_cp method can reflect the real infectious level and provide constant result for a sample within wide range of concentration.

4. No standard required: There is no need to make a calibration curve for Q_cp method. Since a unique ΔΔC_t strategy is introduced to interpret the final result , there will be no waste for standards, as the reagents are only for the use of samples.

to learn about comparison of IQ REAL^TM and IQ2000^TM PCR system (more)