Infectious Myonecrosis Virus Detection and Prevention for Shrimp Farming (IMNV)

Introduction:

Infectious Myonecrosis Virus (IMNV) attacks crops of L. vannamei. The virus originated from Brazi in 2002. Not until February, 2004, that Dr. Donald V. Lightner of the University of Arizona, US, officially confirmed the discovery of the new virus. The late discovery and the insufficiency of information have caused severe damages to the Brazilian farming industry.

Based on some researches, the mortality can reach to 50% starting to aggravate severely from 6 grams. The most visible physical symptom is the appearance of cooked shrimp.

In cooperation with the research team leaded by Dr. Donald V. Lightner, Farming IntelliGene Tech Corp. has created a highly accurate detection system for IMNV, a new product in IQ2000^TM viral detection series. It has inherited all the same features from IQ2000^TM series, such as internal control and semi-quantitative design. The first design eliminates false negative possibilities, and the second one measures the degree of infection. (You can find more information here)

Specifications:

1. Packing size: 200 reactions.

2. Detection Limit: 10 copies/reaction.

3. Quantitative capability: 3 different levels of infection can be differentiated.

4. Sample throughput: for 40 samples, from sample preparation to final results require 4.5 to 5 hours.

5. Built-in internal control system: eliminate false negative results from failed extraction or reaction.

6. Quantified positive standard: monitor the sensitivity of detection.

7. Adaptable to the Uni-IQ RT-PCR profile, suitable for multi-viral diagnosis.

Typical diagnostic results generated by IQ2000^TM IMNV Detection and Prevention System.

Lane 1: IMNV P(+) standard, 2000 copies/reaction

Lane 2: IMNV P(+) standard, 200 copies/reaction

Lane 3: IMNV P(+) standard, 20 copies/reaction

Lane 4: Negative control (yeast tRNA or ddH₂O)

Lane 5: Sample of severe IMNV infection

Lane 6: Sample of light IMNV infection

Lane 7: IMNV negative sample

Lane M: Molecular weight marker, 848 bp, 630 bp, 333 bp

Applications:

• Suitable for fresh, frozen and ethanol preserved shrimp tissue samples, e.g. gill and PL.

• Ideal for SPF animal selection, evaluation of culture environment, grow-out monitoring, and quarantine service.

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