Infectious Hypodermal and Hematopoietic Necrosis Virus Detection and Prevention For Shrimp Farming (IHHNV nested)

Introduction:

Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) has been wildly reported as one of the major viral diseases in shrimp culture industry. It is known to be transmitted both vertically and horizontally. This single strand, negative sense DNA virus can cause mass mortality for L. stylirostris and runt deformity syndrome (RDS) for P. vannamei. Recent research findings pointed out that there is a close correlation between IHHNV and muscle whitening syndrome. Although IHHNV may not affect the growth of P. monodon significantly, however, recent research results also pointed out that it will influence the survival rate of the monodon broodstocks. Thus, the economic loss cause by this virus has been underestimated for a long time.

Dr. Kathy F. J. Tang published the IHHNV-related sequence in the genome of P. monodom from Africa and Australia in late 2006. They developed a PCR method which can be used to differentiate the IHHNV sequence from the IHHNV-related one in 2007. Their findings told there is opportunity of false positive test results if tested by some commercial PCR test kit. After our tests on the P. monodon come from Africa and Australia by our IQ2000^TM IHHNV one step PCR kit, 5% false positive results were found. However, no false positive result is found on P. vannamei.

To rest sure of the test result on P. monodon especially come from Africa and Australia, we developed the IQ2000^TM IHHNV (nested) Detection and Prevention System to fulfill the requirement of the customers who need more accurate and higher bio-security level.

With the new system, not only the false-positive results can be avoided, but also the detection sensitivity will be increased to 10 copies per reaction same as other IQ2000 nested PCR test kits.

Specifications:

 Packing size: 200 reactions.

 Detection Limit: 10 copies/reaction.

 Quantitative capability: 3 different levels of infection can be differentiated.

 Sample throughput: for 40 samples, from sample preparation to final results require 3.5 to 4 hours.

 Built-in internal control system: eliminate false negative results from failed extraction or reaction.

 Quantified positive standard: monitor the sensitivity of detection.

 Adaptable to the Uni-IQ RT-PCR profile, suitable for multi-viral diagnosis.

Typical diagnostic results generated by IQ2000^TM IHHNV nested Detection and Prevention System.

Lane 1: IHHNV P(+) standard, 2000 copies/reaction

Lane 2: IHHNV P(+) standard, 200 copies/reaction

Lane 3: IHHNV P(+) standard, 20 copies/reaction

Lane 4: Sample of severe IHHNV infection

Lane 5: Sample of moderate IHHNV infection

Lane 6: Sample of very light IHHNV infection

Lane 7: IHHNV negative sample

Lane 8: Negative control (yeast tRNA or ddH₂O)

Lane M: Molecular weight marker, 848 bp, 630 bp, 333 bp

Applications:

Suitable for fresh, frozen and ethanol preserved shrimp tissue samples, e.g. broodstock's eye stalk, gill, pleopod, periopod, muscle and PL.

 Ideal for SPF animal selection, evaluation of culture environment, grow-out monitoring, and quarantine service.

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