Infectious Hypodermal and Hematopoietic Necrosis Virus Detection and Prevention For Shrimp Farming (IHHNV)

Introduction:

IHHNV is a notorious pathogen, which can cause mass mortality for L.stylirostris and RDS (runt and deformity syndrome) for L.vannamei. Recent research also points out that IHHNV is related to muscle whitening syndrome and it may affect the survival rate of domesticated P.monodon.

IQ2000^TM IHHNV Detection and Prevention is designed for direct diagnosis of Infectious Hypodermal and Hematopoietic Necrosis Virus. While designing this system, the most conserved region of IHHNV was discovered and used as the target for IHHNV detection. Due to the IHHNV infection is always presented in much higher concentration than other viruses, a single step PCR amplification is sufficient for its detection.

To ensure accurate and reliable results, this system also contains internal control and quantified positive standard. Semi-quantitative designs differentiate 3 different levels of infection; this provides useful information for shrimp farm health management.

Specifications:

1. Packing size: 200 reactions.

2. Detection Limit: 100 copies/reaction.

3. Quantitative capability: 3 different levels of infection can be differentiated.

4. Sample throughput: for 40 samples, from sample preparation to final results require 2.5 to 3 hours.

5. Built-in internal control system: eliminate false negative results from failed extraction or reaction.

6. Quantified positive standard: monitor the sensitivity of detection.

Typical diagnostic results generated by IQ2000^TM IHHNV Detection and Prevention System.

Lane 1: IHHNV P(+) standard, 20000 copies/reaction

Lane 2: IHHNV P(+) standard, 2000 copies/reaction

Lane 3: IHHNV P(+) standard, 200 copies/reaction

Lane 4: Negative control (yeast tRNA or ddH₂O)

Lane 5: Sample of severe IHHNV infection

Lane 6: Sample of moderate IHHNV infection

Lane 7: Sample of light IHHNV infection

Lane 8: IHHNV negative sample

Lane M: Molecular weight marker, 848 bp, 630 bp, 333 bp

Applications:

• Suitable for fresh, frozen and ethanol preserved shrimp tissue samples, e.g. PL, pleopod and gill.

• Ideal for SPF animal selection, evaluation of culture environment, grow-out monitoring, and quarantine service.

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