Macrobrachium rosenbergii Nodavirus Detection and Prevention for Shrimp Farming (MrNV)

Introduction:

Freshwater prawn- Macrobrachium rosenbergii has been viewed as an important species in shrimp farming industry for its high economic value. However, it is under serious threat to a virus borne disease termed white tail disease (WTD).

The typical gross signs of WTD in infected PL are lethargy and opaque abdominal muscles. Mortality reached 100% within 2 to 3 days after the first appearance of prawns with whitish muscles.

The causative agents of this disease are M. rosenbergii nodavirus (MrNV) and extra small virus (XSV). Research result shows that MrNV plays a key role in WTD and that XSV is a satellite virus dependent on MrNV (Zhang et al. 2006).

Until now, WTD was reported in French West Indies, Taiwan, The People’s Republic of China and Thailand.

Though MrNV and XSV failed to cause mortality in marine shrimp, experiment shows that they were tested positive in most organs in P. indicus, P.japonicus and P. monodon (Sudhakaran et al. 2006).

This system has adopted the Nested PCR design and inherited an advantage of IQ2000™, such as reliability and sensitivity. Its unique semi-quantitative function provides information of virus infection levels which could be used for further research on MrNV.

Specifications:

 Packing size: 200 reactions.

 Detection Limit: 10 copies/reaction.

 Quantitative capability: 3 different levels of infection can be differentiated.

 Sample throughput: for 40 samples, from sample preparation to final results require 3.5 to 4 hours.

 Built-in internal control system: eliminate false negative results from failed extraction or reaction.

 Quantified positive standard: monitor the sensitivity of detection.

 Adaptable to the Uni-IQ RT-PCR profile, suitable for multi-viral diagnosis.

Typical diagnostic results generated by IQ2000^TM MrNV Detection and Prevention System.

Lane 1:  MrNV P(+) standard, 2000 copies/reaction

Lane 2:  MrNV P(+) standard, 200 copies/reaction

Lane 3:  MrNV P(+) standard, 20 copies/reaction

Lane 4:  Negative control (ddH₂O)

Lane 5:  Sample of severe MrNV infection

Lane 6:  Sample of moderate MrNV infection

Lane 7:  MrNV negative sample

Applications:

Suitable for fresh, frozen and ethanol preserved shrimp tissue samples, e.g. broodstock's eye stalk, gill, pleopod, periopod and muscle.

 Ideal for SPF animal selection, Evaluation of culture environment, Grow-out monitoring, and Quarantine service.

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